Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Min, J.; Lu, Y.; Hu, X.; Zhou, N.Y.
    Biochemical characterization of 3-methyl-4-nitrophenol degradation in Burkholderia sp. strain SJ98 (2016), Front. Microbiol., 7, 791 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.167 the enzyme is encoded in the pnpABA1CDEF cluster, which is involved in para-nitrophenol (PNP) and 2-chloro-4-nitrophenol (2C4NP) catabolism and is also likely responsible for 3-methyl-4-nitrophenol (3M4NP) degradation in strain SJ98, real-time quantitative PCR enzyme expression analysis, recombinant expression of N-terminally His6-tagged enzyme PnpA in Escherichia coli strain Rosetta(DE3)pLysS Burkholderia sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.167 additional information generation of pnpA deletion mutant SJ981pnpA1, the mutant shows that pnpA is absolutely necessary for the catabolism of 3-methyl-4-nitrophenol Burkholderia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.167 additional information
-
 additional information Michaelis-Menten kinetics Burkholderia sp.
1.14.13.167 0.0203
-
 3-Methyl-4-nitrophenol pH and temperature not specified in the publication Burkholderia sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.167 3-methyl-4-nitrophenol + NADPH + H+ + O2 Burkholderia sp.
-
 2-methyl-1,4-benzoquinone + nitrite + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.6 Burkholderia sp. SJ98
-
-
-
1.14.13.167 Burkholderia sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.167 recombinant N-terminally His6-tagged enzyme PnpA from Escherichia coli strain Rosetta(DE3)pLysS by nickel affinity chromatography Burkholderia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.6 NADPH + H+ + methyl-1,4-benzoquinone
-
 Burkholderia sp. SJ98 NADP+ + methylhydroquinone
-
 ?
1.14.13.167 3-methyl-4-nitrophenol + NADPH + H+ + O2
-
 Burkholderia sp. 2-methyl-1,4-benzoquinone + nitrite + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.167 PNP 4-monooxygenase
-
 Burkholderia sp.
1.14.13.167 pnpA
-
 Burkholderia sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.167 FAD
-
 Burkholderia sp.
1.14.13.167 NADPH
-
 Burkholderia sp.

General Information

EC Number General Information Comment Organism
1.6.5.6 physiological function methyl-1,4-benzoquinone and methylhydroquinone are the intermediates before ring cleavage during 3-methyl-4-nitrophenol degradation, with a nearly stoichiometric formation of methyl-1,4-benzoquinone and methylhydroquinone from 3-methyl-4-nitrophenol Burkholderia sp. SJ98
1.14.13.167 metabolism the enzyme is involved in 3-methyl-4-nitrophenol degradation in Burkholderia sp. strain SJ98, phathway overview. Methyl-1,4-benzoquinone (MBQ) and methylhydroquinone (MHQ), rather than catechol proposed previously, are identified as the intermediates before ring cleavage during 3-methyl-4-nitrophenol degradation by Burkholderia sp. strain SJ98. By sequential catalysis assays, PnpCD, PnpE, and PnpF are likely involved in the further pathway of 3-methyl-4-nitrophenol catabolism Burkholderia sp.
1.14.13.167 physiological function PnpA, a PNP 4-monooxygenase, is able to catalyze the monooxygenation of 3-methyl-4-nitrophenol to 3-methyl-1,4-benzoquinone. PnpB, a 1,4-benzoquinone reductase, has the ability to catalyze the reduction of 3-methyl-1,4-benzoquinone to methylhydroquinone. Moreover, PnpB is also able to enhance PnpA activity in vitro in the conversion of 3-methyl-4-nitrophenol to 3-methyl-1,4-benzoquinone Burkholderia sp.